Self-contained biological indicators (SCBI’s) are available for monitoring the efficacy of steam and ethylene oxide sterilization processes.
SCBI’s contain bacterial spores on a paper carrier plus a sealed glass ampoule within a vented plastic vial.
The glass ampoule contains a proprietary formulation of soybean casein digest culture media with a pH indicator. The pH indicator remains purple when there is no spore growth and yellow when there is spore growth. This allows quick visual determination of sterilization failures (yellow color).
Microorganism |
Sterilization Process |
Incubation time |
Incubation temperature |
Etigam Productcode |
Geobacillus stearothermophilus 10^5 | Steam | 24 hours |
60 °C |
NSC-S5 |
Geobacillus stearothermophilus 10^6 |
Steam | 24 hours | 60 °C | NSC-S6 |
Bacillus Atrophaeus (Subtilis var. Niger) 106 |
E.O. gas |
48 hours |
37 °C |
NSC-E6 |